True resolution
For many years, the study of transcriptome dynamics has been carried with tecnologies such as RNA sequencing and microarrays, just to name a few. These techniques usually require several million of cell to yield a pool of template RNA and be subject of the measurement. This, while being an established, robust and cost-effective experimental set-up, provides an averaged sampling of all transcriptomes across a cell population.
By using stae-of-art technology such those offered by 10X Genomics and Parse Biosciences, IGATech is offering the possibility to move to a new paradigm. The platform is capable of simultaneously tagging hundreds to tens of thousands cell within a single library. After sequencing, reads can be assigned to the original cell and, based on transcriptome profiles, it is possible to distinguish subpopulations, trajectory of development, states as well as to discover associated marker genes. Moreover, oligo-antibody conjugates can be used to selectively tag specific cell types, measure the abundance of certain surface proteins and precisely stratify cells based on their differentiation state.
Lately we also introduced Curio Seeker as our method of choice to deliver Sparial Transcriptomics services.
Cell-plex
When using 10X Genomics products, the basic principle is to tag different samples with specific oligo tags conjugated with non-selective antibodies and then follow standard procedure of sample preparation and sequencing. With Parse bioscience, early segregation in the split-pool barconding process allows to prepare several samples simultaneously. This brings two extremely important benefits:
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All samples are processed in the same experiments removing batch effect.
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A single preparation reagents set is used for several samples, significantly reducing costs.
scRNA-Seq bundle |
Analyzed cells/sample |
Accepted sample type |
Price per sample* |
|---|---|---|---|
| 1 sample standard preparation | 10000 | Frozen, fresh or methanol-fixed | 7450 euro |
1 sample standard preparation |
5000 |
Frozen, fresh or methanol-fixed |
6400 euro |
| 2 samples standard preparation | 5000 | Frozen, fresh or methanol-fixed | 5400 euro |
2 samples with Cell Plex |
5000 |
Frozen, fresh or Paraformaldehyde-fixed (Parse Bioscience) |
4600 euro |
4 samples with Cell Plex |
5000 |
Frozen, fresh or Paraformaldehyde-fixed (Parse Bioscience) |
3800euro |
8 samples with Cell Plex |
5000 |
Frozen, fresh or Paraformaldehyde-fixed (Parse Bioscience) |
2250 euro |
*Pricing are indicative for 25k reads/cell depth and >80% cell viability. Prices can be subject to
modification depending on vendor, selection, protocol, read depth and actual cell viability.
For more detailed quotation or alternative solutions, please enquire.
If you are interested to enter into the single-cell RNA-seq resolution, we are looking forward to help you.
Contact us and plan your experimental set-up with one of our experts.
