Single-cell RNA-Seq

In complex processes such as development, immunology, cancer progression, brain plasticity, the cell heterogenity is known to play a crucial role. Analysis of several individual cells, having distinct fates, interactions and specialized activity is the current standard resolution required to reach new insight.

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Technologies

NovaSeq6000

NextSeq500

Single-cell RNA-Seq

Features

Scalable analysis from hundreds to tens of thousands of cells
Assistance in experimental design and sample management
Cell count and vitality assessment QC
Custom cleaning of dead cells
Cell Plex for cost reduction (See table)
Free basic bioinformatics

True resolution

For many years, the study of transcriptome dynamics has been carried with tecnologies such as RNA sequencing and microarrays, just to name a few. These techniques usually require several million of cell to yield a pool of template RNA and be subject of the measurement. This, while being an established, robust and cost-effective experimental set-up, provides an averaged sampling of all transcriptomes across a cell population.

By adopting 10X Genomics technology, IGATech is offering the possibility to move to a new paradigm. The platform is capable of simultaneously tagging hundreds to tens of thousands cell within a single library. After sequencing, reads can be assigned to the original cell and, based on transcriptome profiles, it is possible to distinguish subpopulations, trajectory of development, states as well as to discover associated marker genes. Moreover, oligo-antibody conjugates can be used to selectively tag specific cell types, measure the abundance of certain surface proteins and precisely stratify cells based on their differentiation state.

NEW: Cell-plex

We tested and validated multi-sample barcoding system. The basic principle is to tag different samples with specific oligo tags conjugated with non-selective antibodies and then follow standard procedure of sample preparation and sequencing. This brings two extremely important benefits:

All samples are processed in the same experiments removing batch effect.
A single preparation reagents set is used for several samples, reducing costs.

scRNA-Seq bundle	Analyzed cells/sample	Accepted sample type	Price per sample*
1 sample standard preparation	10000	Frozen, fresh or methanol-fixed	7450 euro
1 sample standard preparation	5000	Frozen, fresh or methanol-fixed	6400 euro
2 samples standard preparation	5000	Frozen, fresh or methanol-fixed	5400 euro
2 samples with Cell Plex	5000	Frozen or fresh	4000 euro
4 samples with Cell Plex	5000	Frozen or fresh	2600 euro
6 samples with Cell Plex	3000	Frozen or fresh	1900 euro