True resolution
For many years, the study of transcriptome dynamics has been carried with tecnologies such as RNA sequencing and microarrays, just to name a few. These techniques usually require several million of cell to yield a pool of template RNA and be subject of the measurement. This, while being an established, robust and cost-effective experimental set-up, provides an averaged sampling of all transcriptomes across a cell population.
By adopting 10X Genomics technology, IGATech is offering the possibility to move to a new paradigm. The platform is capable of simultaneously tagging hundreds to tens of thousands cell within a single library. After sequencing, reads can be assigned to the original cell and, based on transcriptome profiles, it is possible to distinguish subpopulations, trajectory of development, states as well as to discover associated marker genes. Moreover, oligo-antibody conjugates can be used to selectively tag specific cell types, measure the abundance of certain surface proteins and precisely stratify cells based on their differentiation state.
NEW: Cell-plex
We tested and validated multi-sample barcoding system. The basic principle is to tag different samples with specific oligo tags conjugated with non-selective antibodies and then follow standard procedure of sample preparation and sequencing. This brings two extremely important benefits:
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All samples are processed in the same experiments removing batch effect.
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A single preparation reagents set is used for several samples, reducing costs.
scRNA-Seq bundle |
Analyzed cells/sample |
Accepted sample type |
Price per sample* |
---|---|---|---|
1 sample standard preparation | 10000 | Frozen, fresh or methanol-fixed | 7450 euro |
1 sample standard preparation |
5000 |
Frozen, fresh or methanol-fixed |
6400 euro |
2 samples standard preparation | 5000 | Frozen, fresh or methanol-fixed | 5400 euro |
2 samples with Cell Plex |
5000 |
Frozen or fresh |
4000 euro |
4 samples with Cell Plex |
5000 |
Frozen or fresh |
2600 euro |
6 samples with Cell Plex |
3000 |
Frozen or fresh |
1900 euro |
*Pricing examples for 50k reads/cell depth and >80% cell viability.
For more detailed quotation or alternative solutions, please enquire.
If you are interested to enter into the single-cell RNA-seq resolution, we are looking forward to help you.
Contact us and plan your experimental set-up with one of our experts.