Single-cell RNA-Seq

In complex processes such as development, immunology, cancer progression, brain plasticity, the cell heterogenity is known to play a crucial role. Analysis of several individual cells, having distinct fates, interactions and specialized activity is the current standard resolution required to reach new insight.

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Technologies
NovaSeq6000
NextSeq500
Single-cell RNA-Seq

Features

  1. Scalable analysis from hundreds to tens of thousands of cells
  2. Assistance in experimental design and sample management
  3. Cell count and vitality assessment QC
  4. Custom cleaning of dead cells
  5. Cell Hashing for cost reduction (See table)
  6. Free basic bioinformatics

True resolution

For many years, the study of transcriptome dynamics has been carried with tecnologies such as RNA sequencing and microarrays, just to name a few. These techniques usually require several million of cell to yield a pool of template RNA and be subject of the measurement. This, while being an established, robust and cost-effective experimental set-up, provides an averaged sampling of all transcriptomes across a cell population. 

By adopting 10X Genomics technology, IGATech is offering the possibility to move to a new paradigm. The platform is capable of simultaneously tagging hundreds to tens of thousands cell within a single library. After sequencing, reads can be assigned to the original cell and, based on transcriptome profiles, it is possible to distinguish subpopulations, trajectory of development, states as well as to discover associated marker genes. Moreover, oligo-antibody conjugates can be used to selectively tag specific cell types, measure the abundance of certain surface proteins and precisely stratify cells based on their differentiation state. 

NEW: Cell hashing

We tested and validated multi-sample barcoding system. The basic principle is to tag different samples with specific oligo tags conjugated with non-selective antibodies and then follow standard procedure of sample preparation and sequencing. This brings two extremely important benefits:

 

  • All samples are processed in the same experiments removing batch effect.

  • A single preparation reagents set is used for several samples, reducing costs.

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scRNA-Seq bundle
Total number of cells
Accepted sample type
Project cost
Per sample cost
1 sample
10k
Frozen, fresh or methanol-fixed
5487 euro 5487 euro
2 samples with Cell Hashing
12k 
(6k cells/sample)
Frozen or fresh
7853 euro 3927 euro
4 samples with Cell Hashing
12k 
(3k cells/sample)
Frozen or fresh
8510 euro 2129 euro
8 samples with Cell Hashing 24k 
(3k cells/sample)
Frozen or fresh 14801 euro 1850 euro
Pricing examples for 50k reads/cell depth and >80% cell viability.
For more detailed quotation or alternative solutions, please enquire.
 

If you are interested to enter into the single-cell RNA-seq resolution, we are looking forward to help you.

 

Contact us and plan your experimental set-up with one of our experts.

Documents & Reports

Human samples clearance

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M07 04 Samples Spreadsheet v5.xlsx

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Privacy Information

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Terms and Conditions

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Stoeckius 2018 Cell Hashing.pdf

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