14 March 2023
If you missed it, you can watch our FREE Webinar on demand : A deep characterization of gamma-retroviral transduced human primary keratinocytes integration pattern.
Gene Therapy (GT) achieved remarkable results in treating monogenic diseases deemed incurable by conventional medicine.
05 September 2022
12 July 2022
IGA Webinar - Single-cell transcriptomics: key issues, new perspectives, and current challenges in the field
IGA Live Webinar n.2, was on 11th July, we are sorry we missed you!
11 March 2022
Human DNA contamination in 16S rRNA gene sequence analysis
16S rRNA sequencing is fast and cost-effective approach used to investigate microbiome communities, yet it suffers some inefficiencies. The major problem encountered is eukaryotic host contamination, i.e. >80% of 16S reads can be wasted due to off target amplification from the host’s genome. While this problem is well known to those who work with plants, it seems to be underestimated in the human sector.
24 June 2021
Sustainable Agriculture: Role of Metagenomics in Exploring the Soil Microbiota
The implementation of the EU Farm to Fork Strategy will require an environmentally-friendly utilization of existing natural resources to secure sustainable food production, including a fair use of agricultural land. To reach the 2030 targets, growers will need to change agronomic practices, including soil management, and minimize the use of chemical inputs, the consequences of which on soil microbial communities and plant nutrition are currently unknown.
10 March 2021
IGATech Contest 2020: #scRNAseq Winner Project Presentation
Pleased to present the winning proposal “Understanding tumor and microenvironmental heterogeneity in primary and secondary cancers by single-cell RNA-seq” sent by the 31 y/o post-doctoral research fellow Elena Campaner from the University of Trieste.
05 January 2021
#LabNote: A new solution for low input miRNA-seq
Deep sequencing of entire population of expressed miRNAs in a given sample simultaneously queries thousands of miRNA sequences with extraordinary sensitivity and ability to detect novel ones. In general, the approach is used to give insights in how post-transcriptional regulation contribute to phenotype and for the discovery and development of novel biomarkers for diagnostic testing. In addition, given its extreme sensitivity, miRNA-seq is suitable for detection of both RNA and DNA viruses, as well as viroids that can be missed using targeted detection methods.
25 November 2020
Metabarcoding sequencing: moving from MiSeq to NovaSeq
DNA metabarcoding, i.e. targeted sequencing of taxonomically informative genetic markers, is an efficient method for the assessment and monitoring of biological diversity. Microbiome research is rapidly advancing boosted by sequencing of 16S rRNA gene and ITS region, while the noninvasively surveying plant and animal species richness from different ecosystems is nowadays routinely achieved through the sequencing of plastid or mitochondrial loci such as rbcL, matK and COI or cox1.
21 August 2020
Amplicon-Seq for SARS-CoV-2 detection: tips for improved yields
Since studies related to COVID-19 are attracting great scientific interest, we recently focused our activities in recovery of viral sequences from swab samples by two methods: amplicon-Seq and RNA-Seq (using Trio RNA-Seq library preparation kit by Tecan Genomics as reported in our previous post).
21 July 2020
Ultra-low RNA-seq for SARS-CoV-2 detection from nasopharyngeal swabs
critical factors to consider for deep sequencing from clinical samples
30 April 2020
Exome-Seq: #Quality #Genetics
Proud to announce that we are keeping our quality standards high by qualifying again above the mean in the external quality assessment of NextGen DNA Sequencing by the European Molecular Genetics Quality Network (EMQN).
22 April 2020
Applying RNA-seq to COVID19 clinical samples
Sequencing of the SARS-Cov2 genomes using standard RNA-Seq strategies has been so far mainly achieved using viral culture or BALF, providing adequate viral copies as input template. However, for large-scale surveys of genome mutations, their spread, along with the ability to assess potential co-infections, the analysis of common swab samples with an unbiased RNA-Seq approach is an ideal solution, potentially, for any respiratory infection.
27 January 2020
Exome-seq saturation analysis: when you should STOP sequencing
Sequencing coverage in exome-seq is one of the major issues since a small difference in percentage coverage may significantly impact diagnostic outcome.
06 August 2019
Exome-Seq: dealing with degraded samples
It is known that Next Gen sequencing requires the gDNA input of high molecular weight to construct quality libraries.
20 June 2019
NeosVine: a pioneering project for sustainable viticulture
Genome editing for improved vines The effects of climate change and population growth open new scenarios for agriculture and set new goals for researchers and farmers. In fact, today’s increase in agricultural production cannot be separated from the reduction of its environmental impact. In this perspective, green biotechnology research is focused on studying plant genomes to improve adaptability and resistance to different environmental conditions and pathogens/parasites, without altering the genetic characteristics of the cultivated varieties. The goal is to grow stronger and more adaptive plants and enable farmers to become less dependent on chemicals to protect crops. The picture just described is the focal point of the NeosVine research project, started by IGA Technology Services, a spin-off of the Institute of Applied Genomics of Udine (IGA) in collaboration with the Vivai Cooperativi Rauscedo, granted by the Italian Autonomous Region Friuli Venezia Giulia.
22 May 2019
Proud to announce that we have achieved ISO 9001:2015 Certification
Proud to announce that we have achieved ISO 9001:2015 Certification, an internationally recognized quality management standard that guaranties our ability to consistently provide services that meet both customer and applicable statutory and regulatory requirements.
20 March 2019
Universal primers for characterization of fungal microbiome in higher plants
Just a few days ago we discussed difficulties encountered when investigating host-associated mycobiomes by a metabarcoding approach suggesting some solutions.
17 December 2018
Buffalo or Cow? Metagenomic profiles of different types of Italian high-moisture Mozzarella cheese
In our latest study we compared metagenomic profiles of different mozzarella cheeses.
06 August 2018
NeosVine Project: Green biotechnologies to improve the sustainability of viticulture & tackle climate change
Global agriculture under pressure: increasing output while minimizing environmental impact Incredible population growth (by the middle of the 21st century we will be over nine billion people) imposes an unprecedented demand on the planet’s resources and capabilities. Imbalance between the agricultural lands needed for food production and the boost urban development, needed to accommodate this population increase, goes at the expense of farm lands. Consider also climatic changes that are likely going to have a negative impact on both natural ecosystems and agricultural productivity. The picture is quite dramatic.