21 May 2018
Targeted capture is one of the most popular NGS applications. However, due to the often-limited amounts of tissue it is challenging to satisfy quality requirements and obtain 200 ng of input DNA still needed by most protocols.
23 April 2018
Metabarcoding Identification and classification of eukaryotes increasingly depends on DNA sequences of standardized genetic markers, a concept known as DNA barcoding. Thus, molecular taxonomists continue to search for regions conserved enough to allow reliable priming but sufficiently variable to yield highly resolved and well-supported phylograms. The ideal characteristics include adequate species resolution, ease of amplification, absence of extreme length variation, the presence of only single copies and low intra-species variability.
13 April 2018
ChIP-Seq is extensively used to characterize genome-wide binding patterns of transcription factors and other chromatin-associated proteins.
26 March 2018
So proud to announce that we qualified above the mean and got a green label in the external quality assessment of NextGen DNA Sequencing by European Molecular Genetics Quality Network (EMQN).
18 December 2017
WATBIO is a European Framework 7 funded collaborative research project focused on developing drought-tolerant biomass crops for Europe. IGATech is the leading SME partner in WATBIO and conducts all the next-generation sequencing work. A large proportion of the WATBIO DNA and RNA sequencing work is done on poplar, and the WATBIO project has enabled IGATech to drive significant innovation in the type of sequencing involved.
16 October 2017
#LabNote: implementing PRO-amp - a new protocol that increases the overall quality of 16S sequencing
16S amplicons loaded on a single Illumina lane (regardless of platform type or sequencing length) use to suffer poor quality due to low base complexity. Each cluster on a flowcell starts with the same base (the primers used for amplification) and mostly continue in a low complexity region depending on the diversity of the microbial community (which is anyhow far from balanced base composition on each cycle of sequencing; Fig. 1A). This causes impairing in the cluster identification during first cycles of sequencing since all clusters will emit the same color and thus reduce the ability to discriminate those that juxtapose one another.
23 July 2017
#LabNote: 16S rRNA metagenomics - increasing efficiency by blocking amplification of contaminating sequences from a eukaryotic host
16S rRNA sequencing is the most common approach used to identify microbes without the need to sequence entire genome. It is fast, cost-effective and flexible; however the method suffers some inefficiencies.
04 July 2017
IGATech is keeping active in the human genomics field. After the D.NAMICA project in which we applied Whole Exome Sequencing to better understand the genetic characteristics of patients suffering dilatative cardiomyopathy, hepatocellular carcinoma and spinal muscular atrophy, we are moving to the next level by implementing Whole Genome Sequencing to redefine the diagnosis in breast cancer and in patients with behavioral, cognitive and developmental disorders.
10 May 2017
χ-scan: a method to detect mosaic structural variation by examining deviations in SNP relative allele frequencies
Our latest work addresses the need for a method specifically developed for mosaic structural variants (SV) detection from Next Gen sequencing (NGS) data.
22 March 2017
We finally made it! A brand new - featured - website. We hope this will provide ease of access to all the relevant information regarding our services for loyal and future customers. We thank infoFACTORY for the patient support in the development of the website and graphic creations.