Genotyping by sequencing solutions
by IGA Tech

Overcoming the need for expensive and labor-intensive array-based solutions, the redundancy of sequenced reads is used to generate accurate genotyping across large batches of individuals in a single sequencing run.

Optimal solutions - solid outputs

Animal and plant breedingPlant and animal breeding

Use genomic information on crops, seeds and livestock to make informed selection and breeding decisions. Screening and prediction of useful or disease-linked traits help to make crucial decisions on the selection and health of the population.

Biodiversity and nature conservationBiodiversity and nature conservation

Investigate different organisms in their natural habitat, determine the biodiversity of a territory and evaluate the effect of anthropogenic activities Robust genomic data help to trace migration patterns and aid in the regional and international management of species.

Food industryFood industry

Use genomic data to assess and certify quality, origin and safety of ingredients. Implement innovative traceability strategies that permit sensitive detection of biological contamination and ensure the integrity of any food substrate from raw material to packaged products.

How do we do it? Reducing the complexity

Examine the same subset of genomic regions for thousands of individuals and identify many genetic markers along the genome with the two of the most effective strategies: ddRAD and Allegro SPET Targeted Genotyping.

Plant and animal breeding, ecology and association studies are usually large-scale experiments with massive amount of genetic information to sample and analyze. In these cases, whole genome sequencing remains an unaffordable option. One of the most effective ways to reduce costs is to reduce the genome to a fraction of its size before sequencing without losing precious information.

Double digest RAD Sequencing

A technique uses two restriction enzymes to reduce the complexity of genomes allowing to genotype several thousands of loci. Open system for unbiased sampling of genetic variation also in non-referenced organisms.

Allegro Targeted genotyping

Single primer enrichment technology patented by NuGEN, developed to specifically target SNPs of interest in large scale genotyping experiments. Delivers uniformly enriched genomic features of organisms with reference sequence.

Why IGATech genotyping solutions are the right choice for you?

IGATech has successfully conducted many genotyping experiments on both plants and animals, with genome sizes ranging from hundreds of megabases to tens of gigabases. We pioneered the implementation of the Allegro custom panel in agrigenomics for large scale genotyping

  • Competitive costs. Develop thousands of genetic markers quickly and effectively.
  • Solid Data. More than 95% of sequencing data in loci with adequate overall coverage.
  • Tailored consultancy. From project design to follow-up we want to make sure that the outcome will meet your expectations.
  • No reference sequence needed. Genotype organisms that have never been sequenced before.
  • Open approach. Sample genetic variability without bias also in genetic backgrounds not exhaustively represented in an array or any previous experiment.

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