RNA-Seq

Our continuous commitment to follow and test the latest protocols and tools, as well as to develop new ones, assures the implementation of the state-of-the-art procedures and the optimization of the experimental design. A quality-centric RNA-Seq service.

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Technologies
NovaSeq6000
NextSeq500
Nucleic acids extraction
RNA-Seq

Features

  1. Stranded libraries
  2. rRNA depletion
  3. Depletion of unwanted transcripts
  4. UMI-tagged libraries
  5. Ultra-low input
  6. Full-length cDNA (Oxford Nanopore)
  7. Direct RNA (Oxford Nanopore)

Supported Analyses

  1. Differential expression 
  2. Non-coding RNA analysis
  3. De novo assembly of transcripts
  4. Allelic-specific expression analysis
  5. SNP discovery
  6. GSEA / Clustering analysis

 

 

We’ve been working with IGATech and we’re extremely satisfied. I would suggest IGATech to any group who wants more than a sequencing facility, rather looking for a research partner to be involved in each step of your projects, from experimental design to data analysis.

Raffaele Teperino, PhD
Head of Environmental Epigenetics
Helmoholtz Center, Munich (Germany)

 

One MOLECULE, INFINITE TOOL

Targeted depletion of unwanted transcripts results in a significant reduction in sequencing reads derived from cytoplasmic and mitochondrial rRNAs, globin, chloroplasts, housekeeping genes, or any other transcript species that may not be relevant to a study, for more efficient use of sequencing resources.

As a part of our standard procedure we prepare strand oriented RNA-seq “libraries” that maintain the information on which strand the original mRNA template is coming from, allowing to accurately determine gene expression from overlapping genes or to discover antisense regulators.

 

Tools for efficient cDNA generation from ultra-low inputs supporting research in areas previously hampered by quantity are part of our portfolio.


For the high-throughput single cell RNA-Seq we use 10x Genomics Chromium System that permits to perform deep profiling of heterogeneous populations with digital gene expression on a cell-by-cell basis ensuring that biologically relevant signals of individual cells are not masked by bulk average measurements.

Long-reads sequencing platform (Nanopore) is used in the context of projects where full lenght transcripts are sequenced to obtain fine structure of iso-form to study their differential expression ot to fine annotate de novo assembled genomes. This can be achieved either via sequencing of amplified full-length cDNA in a multiplexed fashion or access the RNA sequence directly with the RNA004 kit from Nanopore (including post-trascriptinal epigenetic modifications).

 

Analysis of RNA-binding protein immunoprecipitation that maps RNA-protein associations in vivo by discovering genome-wide RNA molecules of any type that physically interact with a regulatory protein or protein complex. This as many other custom bioinfomatics analyses can be performed on request. Please enquire

We also provide full support on study design to ensure correct sequencing and bioinformatics strategies are used to meet your project goals. Our expert will consult with you about your specific requirements, and will also be your technical resource and point of contact for the length of your project. See bioinformatics.

 
TAILORED CONSULTANCY

For every project we want to make sure that the outcome will meet your expectations

RNA-Seq bundle
Samples number
Read length
List price per sample
mRNA stranded library
+ 60M paired reads (30M frags)
96+
150bp
189 euro
24+ 220 euro
total RNA-Seq library
+ human/mouse/rat rRNA depletion
+ 60M paired reads (30M frags)
24+
150bp
312 euro
12+ 375 euro
ultra-low (pg) input
stranded totalRNA-Seq*
+ 60M paired reads (30M frags)
*Human/mouse/drosophila and custom depletion available on request.
24+
150bp + UMI
418 euro
12+ 516 euro
Nanopore full-length cDNA (mRNA) 50M transcripts/run (multiplex) 12plex (4M/sample) full-length transcripts 262 euro
Nanopore Direct RNA 1 full-length transcripts 2531 euro

 

Pricing guidelines. For more detailed quotation or alternative solutions please enquire.
 

Documents & Reports

Human samples clearance

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Terms and Conditions v04.pdf

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Corporate.pdf

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Privacy Information

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Samples Spreadsheet v7.xlsx

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Shipping instructions.pdf

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RNA and smallRNA Sample preparation guidelines v6.pdf

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RNA Data delivery specifications.pdf

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Transcriptomics.pdf

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