RNA-Seq
Technologies
Features
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1
Stranded libraries
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rRNA depletion
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Depletion of unwanted transcripts
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UMI-tagged libraries
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Ultra-low input
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6
Full-length cDNA sequencing with Nanopore
Supported Analyses
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1
Differential expression
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Non-coding RNA analysis
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De novo assembly of transcripts
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Allelic-specific expression analysis
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SNP discovery
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6
Clustering analysis
We’ve been working with IGATech and we’re extremely satisfied. I would suggest IGATech to any group who wants more than a sequencing facility, rather looking for a research partner to be involved in each step of your projects, from experimental design to data analysis.
One MOLECULE, INFINITE TOOL
Targeted depletion of unwanted transcripts results in a significant reduction in sequencing reads derived from cytoplasmic and mitochondrial rRNAs, globin, chloroplasts, housekeeping genes, or any other transcript species that may not be relevant to a study, for more efficient use of sequencing resources.
As a part of our standard procedure we prepare strand oriented RNA-seq “libraries” that maintain the information on which strand the original mRNA template is coming from, allowing to accurately determine gene expression from overlapping genes or to discover antisense regulators.
Tools for efficient cDNA generation from ultra-low inputs supporting research in areas previously hampered by quantity are part of our portfolio.
Full-length cDNA (Oxford Nanopore) is used in the context of projects where full length transcripts are sequenced to obtain fine structure of isoforms and to study their differential expression or to fine annotate de novo assembled genomes.
Direct RNA Sequencing: direct RNA sequencing, powered by Oxford Nanopore Technologies provides a direct window into the viral genetic material, bypassing traditional limitations and offering a comprehensive view of viral evolution, mutation rates, and pathogenicity mechanisms. Ideal for emerging pathogens and rapidly mutating viruses this technology also allow to detect base modifications on the RNA molecule such as N6-methyladenosine (m6A) and pseudouridine ( Ψ ) which are now to play important role in stability of the molecule and replication rate. These same modifications are crucial for the development of RNA-based vaccines.
We also provide full support on study design to ensure correct sequencing and bioinformatics strategies are used to meet your project goals. Our expert will consult with you about your specific requirements, and will also be your technical resource and point of contact for the length of your project. See bioinformatics.