---
title: "smallRNA-Seq"
search_description: "IGATech's small RNA sequencing service delivers accurate profiling of miRNA, siRNA, and other small RNA species, supporting research in gene regulation, epigenetics, and disease mechanisms. Enhance your insights with our precise sequencing solutions."
published: 2025-02-10 13:00:59.156739+00:00
locale: "en"
---

# smallRNA-Seq

[Request a quote](https://igatechnology.com/igatech/contacts/#contact-form)

## Technologies

![NovaSeq X Plus](/media/images/Illumina_NovaSeq_XPlus_Left_561x375.width-500.png)

### NovaSeq X Plus

The NovaSeq X Plus is a powerful and scalable ultra-high-throughput sequencing system that supports the broadest range of applications and study sizes. Unrivaled data quality using Illumina's proven SBS chemistry has made the NovaSeq X Plus the instrument of choice for all major genome centers and leading institutions throughout the world. Producing up to 25 billion reads \(7.5 Tbp\) per flowcell, yielding up to 120 human genomes in about 2 days at full capacity.

## Understanding the role of non-coding RNA

**Capture the complete range of small RNA and miRNA species**

Small noncoding RNAs are involved in gene silencing and post-transcriptional regulation of gene expression. Deep sequencing of entire population of expressed small RNAs in a a given sample simuntaneously queries thousands of small RNA and miRNA sequences with extraordinary sensitivity and dynamic range giving insight in how post-transcriptional regulation contributes to phenotype. In addition, smallRNA-seq can be efficiently used for virus identification and discovery. The approach is highly sensitive and detects RNA viruses, DNA viruses and viroids that can be missed using targeted detection methods, such as ELISA or PCR-based tests.

**Identify novel biomarkerS**

### Library preparation and sequencing

Our workflow can start with isolated total RNA or enriched small RNA. Upon the quality control of the starting material we generate libraries and select size in a range that maximizes the number of relevant reads. Sequencing is performed on NextSeq500 in a 75 bp single-read mode. Our establisehd protocol has provided successful library generation also on limiting templates and cumbersome samples \(e.g. serum, exosomes, tree plants\).

![mirna\_heatmap](/media/images/mirna_heatmap.width-1500.png)

### bioinformatics analysis

**Standard bioinformatics analysis includes**:

- Base calling and demultiplexing
- Trimming \(removing lower quality bases and adapters\)
- Count occurrence of each unique sequence \(tag\)
- Size distribution of tags creating a histogram of sequence size. The graph gives a first summary statistics on size distribution of the small RNAs sequences produced by the sequencing run
- Classification of tags and identification of noncoding RNA types
- Exact matching and counting of each miRNA and piRNA in a given sample - mapping reads against [miRBase](http://www.mirbase.org/) and [piRNA](http://pirnabank.ibab.ac.in/request.html) entry
- Prediction of novel miRNAs
- miRNA target prediction
- Pair-wise differential expression analysis to identify known and novel microRNAs that are differentially expressed between sample groups
- Principal Component Analysis \(PCA plot\)

**Advanced bioinformatics analysis includes**:

- Virus identification and discovery

For every project we want to make sure that the outcome will meet your expectations.

If required we can perform **RNA isolation** from a range of samples including cells, tissues and biofluids.

We also provide full support on study design to ensure correct sequencing and bioinformatics strategies are used to meet your project goals. Our expert will consult with you about your specific requirements.

## Terms and Conditions

- [Privacy Information.pdf](/documents/12/Privacy_Information_rev00.pdf)
- [Human samples clearance.pdf](/documents/13/__Human-samples_clearance.pdf)
- [Shipping and Packaging Guidelines.pdf](/documents/9/SHIPPING_AND_PACKAGING_GUIDELINES.pdf)
- [rev05 Terms and Conditions.pdf](/documents/137/__M12_01_rev05_Terms_and_Conditions.pdf)