--- title: "Ribosome profiling" search_description: "IGATechnology’s ribosome profiling services provide high-resolution insights into protein synthesis and translation dynamics, supporting research in gene regulation, cellular biology, and translational control. Unlock precise protein data with our advanced profiling solutions." published: 2025-08-05 10:07:33.023763+00:00 locale: "it" --- # Ribosome profiling We provide an innovative solution for ribosome profiling using a fast and selective extraction of ribosomes in active translation \(both in vitro and in vivo\) and purification of ribosome protected fragments \(RPFs\). Adopting this new technology may be the key to unlocking new insights into the regulation of ribosomes and changes in the translatome that you've been looking for. [Request a quote](https://igatechnology.com/igatech/contacts/#contact-form) ## Technologies ![NovaSeq X Plus](/media/images/Illumina_NovaSeq_XPlus_Left_561x375.width-500.png) ### NovaSeq X Plus The NovaSeq X Plus is a powerful and scalable ultra-high-throughput sequencing system that supports the broadest range of applications and study sizes. Unrivaled data quality using Illumina's proven SBS chemistry has made the NovaSeq X Plus the instrument of choice for all major genome centers and leading institutions throughout the world. Producing up to 25 billion reads \(7.5 Tbp\) per flowcell, yielding up to 120 human genomes in about 2 days at full capacity. ![RNA-Seq](/media/images/RNA-Seq.width-500.png) ### RNA-Seq NGS platforms have become the most convenient platforms to perform accurate quantification of transcripts. Moreover, relying on sequencing information, it is possible to obtain non-trivial information such as mutation in coding regions, alternative splicing with isoforms expression levels, allelic-specific expression, intron retention, etc. Also, when performing *de novo* reconstruction of a genome, RNA-Seq data will be one of the major drivers for gene prediction and modeling. See [RNA-Seq](https://igatechnology.com/genomics-research-services/plantanimal/rna-seq/) - [smallRNA-Seq](https://igatechnology.com/genomics-research-services/small-rna/) ![AVITI Element Biosciences](/media/images/0.width-500.png) ### AVITI Element Biosciences The AVITI System by Element Biosciences is a benchtop sequencer designed for high performance, cost efficiency, and flexibility. It features dual independent flow cells for parallel or independent operation, enabling flexibility in throughput from low to full. The system utilizes Avidity Sequencing™ technology, ensuring over 90% of reads exceed Q30 quality, with minimal errors, low duplication rates, and reduced AT/GC bias. It supports easy data conversion to FASTQ files, offers compatibility with numerous assays, and provides cost-effective sequencing. This is our ideal companion for dedicated and fast-turnaround run set-up. For more detailed information, you can visit their website directly at [https://www.elementbiosciences.com/products/aviti](https://www.elementbiosciences.com/products/aviti). ## Features 1. Only active ribosomes captured 2. Positional data of active ribosomes with nucleotide resolution 3. Rapid protocol to minimize biases ## Services 1. Library preparation 2. In-process QC 3. Dedicated 100bp lanes 4. Custom bioinformatics ![Ribosome](/media/images/Ribosome_.width-1500.png) ## Exploring the Untold Tales of Translation **Ribosome profiling**, also known as **ribo-seq**, is a **powerful experimental technique**that provides **insights into the translation dynamics and protein synthesis**occurring within a cell. A significant portion of our **understanding of gene regulation is derived from research on mRNA levels**, even though protein abundance has a greater influence and post-transcriptional regulation is widespread. **Comprehending the molecular fundamentals of regulatory divergence** within and between species may be highly advantageous. Most of the recognized human ailment-risk alleles are present in non-coding parts of the genome, implying that they have an influence on gene regulation. **By combining ribosome profiling with mRNA sequencing data**, researchers can gain a **more comprehensive understanding of gene expression regulation**, identify translationally regulated genes, discover novel translation events, and explore the dynamics of protein synthesis within cells. ![translation efficiency](/media/images/translation_efficiency.width-1500.jpg) ### **If you are interested to enter the ribosome-profiling challenges, we are looking forward to help you.**[**Contact us**](https://igatechnology.com/igatech/contacts/)**and plan your next experiment with.** ### Relevant publications **Deciphering the immunopeptidome in vivo reveals new tumour antigens. Nature** volume 607, pages149–155 \(2022\) [https://doi.org/10.1038/s41586-022-04839-2](https://doi.org/10.1038/s41586-022-04839-2) **SMN-primed ribosomes modulate the translation of transcripts related to spinal muscular atrophy. Nat Cell Biol**. 2020 Oct;22\(10\):1239-1251. DOI: [10.1038/s41556-020-00577-7](https://doi.org/10.1038/s41556-020-00577-7) **Active Ribosome Profiling with RiboLace. Cell Reports** 25, \(2018\) 1097–1108 DOI: [10.1016/j.celrep.2018.09.084](https://doi.org/10.1016/j.celrep.2018.09.084) **The science of puromycin: From studies of ribosome function to applications in biotechnology. Comput Struct Biotechnol J**. 2020 Apr 24;18:1074-1083. DOI: [10.1016/j.csbj.2020.04.014](https://doi.org/10.1016/j.csbj.2020.04.014) ## Terms and Conditions - [Privacy Information.pdf](/documents/12/Privacy_Information_rev00.pdf) - [Human samples clearance.pdf](/documents/13/__Human-samples_clearance.pdf) - [Shipping and Packaging Guidelines.pdf](/documents/9/SHIPPING_AND_PACKAGING_GUIDELINES.pdf) - [rev05 Terms and Conditions.pdf](/documents/137/__M12_01_rev05_Terms_and_Conditions.pdf)