---
title: "Nanopore Sequencing"
search_description: "Discover IGATech's long-read technology based on Oxford Nanopore, for a comprehensive analysis of structural variants and epigenetic modifications on native nucleid acids."
published: 2026-05-22 12:15:24.845189+00:00
locale: "it"
---

# Nanopore Sequencing

[Contact us!](https://igatechnology.com/igatech/contacts/#contact-form)

## Technologies

![PromethION](/media/images/P24.width-500.png)

### PromethION

Oxford Nanopore's technology is providing unprecedented accessibility and scalability to long-read sequencing. The technology has  proven to be a game-changer in the fields of genome assembly, pangenomics, direct RNA sequencing and metagenomics analyses. Reads can be as long as input DNA/RNA. Bacterial genomes can be assembled in a single molecule in a snap of sequencing. Repetitive elements are easily resolved and with error-correction softwares we can now achieve telomere-to-telomere genome assemblies.

![Nanopore Sequencing](/media/images/1.width-500.jpg)

### Nanopore Sequencing

Nanopore sequencing is empowering many applications by the ability to analyze DNA, RNA and their modifications with no limitation on sequence length. This is of particular importance when the goal is to resolve hard-to-sequence or repetitive regions that have so far hampered the possibility to decode structural and epigenetic variation. Also, direct RNA sequencing and full-length cDNA sequencing are providing unprecedented insight into the real strucure and abundance of transcripts isoforms.

## Advantages

1. Long - read of native DNA and RNA
2. De novo genome assembly
3. Detection of RNA splicing isoforms
4. Adaptive sampling on regions of interest

## Specialized Bioinformatics

1. Genome browser hosting
2. Detection of SNVs, SVs, STRs, CNVs
3. Haplotype phasing

## Provides combined genomic and epigenomic information from native DNA sequencing

**LONG-READ**

Oxford Nanopore sequencing is a **long-read, real-time sequencing technology** that enables the analysis of DNA and RNA molecules without the need for amplification or fragmentation. This approach allows the study of **native nucleic acids** while preserving epigenetic modifications, providing a more comprehensive view of the genome and transcriptome. This is particularly relevant for research on rare diseases and complex genomic regions.

![minion\_animation\_still\_18](/media/images/minion_animation_still_18.width-1500.png)

**Key advantages for human genomics:**

- **Unrestricted read lengths**: N50 exceeding tens of kilobases, enabling the resolution of complex genomic regions like structural variants \(SVs\), repeats, and full-length transcript isoforms
- **Telomere-to-telomere** genome reconstruction
- **Direct detection of** **DNA** **methylation** \(4mC, 5mC, 5hmC, 6mA\)
- **Direct detection of RNA modifications** \(m5C, m6A, pseudouridine, 2’O‑methylations\)
- **Accurate phasing and variant calling**, including SNVs, SVs, STRs, CNVs
- **Adaptive sampling** for targeted sequencing of specific genomic regions
- **Real-time and scalable sequencing**, suitable for large cohorts and translational research

**Applications in Human Genome Research:**

- **Whole Genome Sequencing \(WGS\)**: complete genome coverage, including regions typically missed by short-read technologies
- **Long-read sequencing of human transcriptome**: full-length RNA sequencing allows for isoform characterization and quantification, revealing transcriptome complexity
- **Epigenomics**: haplotype-specific methylation information highlights the impact of maternal vs paternal chromosomes on gene function and phenotypic traits
- **Study of rare diseases** where haplotype-specific variants play a crucial role
- **Metagenomics** and characterization of human microbiome from patients' biological samples \(leveraging the host depletion mode\)

**end-to-end solutions**

We offer end-to-end solutions, including DNA extraction and sample preparation services. In addition, we provide full support in study design to ensure that appropriate sequencing and bioinformatics strategies are applied to meet your project goals. Our experts work closely with clients to define the most effective approach.

## Documents & Reports

- [Privacy Information.pdf](/documents/12/Privacy_Information_rev00.pdf)
- [Human samples clearance.pdf](/documents/13/__Human-samples_clearance.pdf)
- [Shipping and Packaging Guidelines.pdf](/documents/9/SHIPPING_AND_PACKAGING_GUIDELINES.pdf)
- [rev05 Terms and Conditions.pdf](/documents/137/__M12_01_rev05_Terms_and_Conditions.pdf)