--- title: "Nanopore phased-methylation" search_description: "IGATech’s nanopore methylation sequencing enables real-time DNA methylation analysis with long-read technology, supporting epigenetics research, cancer studies, and gene regulation insights. Achieve detailed methylation profiling with our advanced nanopore solutions. Nanopore sequencing service made with excellence." published: 2025-12-12 08:38:28.777936+00:00 locale: "it" --- # Nanopore phased-methylation [Request a quote](https://igatechnology.com/igatech/contacts/#contact-form) ## Technologies ![PromethION](/media/images/P24.width-500.png) ### PromethION Oxford Nanopore's technology is providing unprecedented accessibility and scalability to long-read sequencing. The technology has proven to be a game-changer in the fields of genome assembly, pangenomics, direct RNA sequencing and metagenomics analyses. Reads can be as long as input DNA/RNA. Bacterial genomes can be assembled in a single molecule in a snap of sequencing. Repetitive elements are easily resolved and with error-correction softwares we can now achieve telomere-to-telomere genome assemblies. ![Whole Genome Sequencing](/media/images/Whole_genome_sequencing.width-500.png) ### Whole Genome Sequencing The whole genome sequence as the most comprehensive overview of its composition. Identify most of the variability accounted by SNPs, CNVs, PAVs, insertions, deletions, translocations along with its functional impact on the genetic code an organism. Dedicated technologies are applied for *de novo* reconstruction of personal genomes to an affordable price, providing an unbiased method to depict each individual human \(and non-human\) genome. See [*de novo*](https://igatechnology.com/genomics-research-services/?area=plant#denovo) - [whole genome resequencing](https://igatechnology.com/genomics-research-services/whole-genomics-resequencing/). ![Nanopore Sequencing](/media/images/1.width-500.jpg) ### Nanopore Sequencing Nanopore sequencing is empowering many applications by the ability to analyze DNA, RNA and their modifications with no limitation on sequence length. This is of particular importance when the goal is to resolve hard-to-sequence or repetitive regions that have so far hampered the possibility to decode structural and epigenetic variation. Also, direct RNA sequencing and full-length cDNA sequencing are providing unprecedented insight into the real strucure and abundance of transcripts isoforms. ## One experiment - many results 1. Accurately call SNPs 2. Unprecedented precision in analysis of structural variants 3. Define megabase-scale haplotype-blocks 4. Achieve haplotype-specific methylation 5. Methylation calling in all-context ## Specialized bioinformatics 1. EPI-haplotype reconstruction 2. Methyl and hydroxy-methyl base calls 3. Define methylation at and adjacent insertion event 4. Genome browser hosting ## The genomics and epigenomics in one stop shop **ADAPTIVE SAMPLING** It can enable the in-sequencing enrichment of target regions of interest to enhance the coverage and provide more cost-effective experiments The unprecedented ability of Nanopore technology to read native DNA molecules unleash the potential to analyze the genetic and epigenetic code in one single experiment without interfering with the starting DNA template. Molecules of >50Kb can be sequenced to access base content and epigenetic modifications with >99% accuracy. **Superior coverage** Thanks to PCR-free preparation and long-reads mapping the a highly homogeneous coverage is achieved without drop in repetitive regions which are usually lost in short-read sequencing. Also centromeric regions can be spanned. ![P24-light](/media/images/P24_FP5WKtp.width-1500.png) ### ​Explore Advanced Capabilities with IGATech's Nanopore Sequencing Services IGATech offers Nanopore Sequencing services to provide detailed insights into DNA and RNA sequences. This service is pivotal for comprehensive methylation analysis and haplotype reconstruction, enabling researchers to explore genomics landscapes with native data integration.. **High-Accuracy Methylation Analysis Across All Cytosine Contexts** With Nanopore Sequencing we can deliver base-by-bae methylation level across all cytosine \(CG, CHG, CHH\). This high level of precision is crucial for understanding the intricate regulatory mechanisms influenced by epigenetic modifications. The ability to map long reads also in repetitive regions and maintaining unaltered cytosines \(unlike bisulfite or enzymatic conversion of cytosine to T\) allow to maintain an extremely high quality of unambiguous mapping. Complete and even coverage for hard-to-sequence regions such as mobile elements, centromeres, tandem repeats is the the only approach to complete the picture. **Advanced Bioinformatics Support** IGATech provides advanced bioinformatics support to assist clients in identifying differentially methylated sites and regions, tailoring analyses to specific research objectives. This ensures the extraction of actionable insights from extensive methylation data, including the contextual genomic methylation data across both haplotypes whose phases have been accurately reconstructed thanks to the long reads offered by this technology. **Haplotype-Specific Methylation Information** Our service uniquely resolves haplotypes in diploid individuals, providing haplotype-specific methylation information. This capability allows researchers to examine how methylation patterns differ across chromosomes and assess their impact on gene function and phenotypic traits. ![ONT-meth](/media/images/ONT-meth.width-1500.png) Haplotype-resolved structural variation \(Copia-like transposable element insertion on track 1\) and associated variation in the methylation pattern **Adaptive Sampling for Targeted Sequencing** IGATech's Nanopore Sequencing also supports adaptive sampling, enabling targeted enrichment of sequencing efforts in regions of high biological interest, such as gene families and their promoters. This feature makes experiments more cost-effective by allowing the sequencing of more samples on the same flow cell, maximizing the utility and efficiency of sequencing runs. **end-to-end nanopore sequencing service** We also offer DNA extraction sample preparation service and provide full support on study design to ensure correct sequencing and bioinformatics strategies are used to meet your project goals. Our expert will consult with you about your specific requirements. ## Terms and Conditions - [Privacy Information.pdf](/documents/12/Privacy_Information_rev00.pdf) - [Human samples clearance.pdf](/documents/13/__Human-samples_clearance.pdf) - [Shipping and Packaging Guidelines.pdf](/documents/9/SHIPPING_AND_PACKAGING_GUIDELINES.pdf) - [rev05 Terms and Conditions.pdf](/documents/137/__M12_01_rev05_Terms_and_Conditions.pdf)