--- title: "Metatranscriptomics" search_description: "IGATech’s metatranscriptomics services provide in-depth insights into active microbial communities by analyzing gene expression in complex environments, ideal for microbiome research, ecology, and environmental genomics. Discover functional dynamics with our advanced sequencing solutions." published: 2025-02-10 13:01:58.941943+00:00 locale: "it" --- # Metatranscriptomics [Contact us](https://igatechnology.com/igatech/contacts/#contact-form) ## Technologies ![Nucleic acids extraction](/media/images/DNA_extraction.width-500.png) ### Nucleic acids extraction DNA and RNA extraction is routinely performed at IGATech, providing support to our customers directly from raw material. Relying on automated systems and consolidated protocols we can help you in accelerating the path to analyzed data. We also work with non standard substrates, such as [plants](https://igatechnology.com/genomics-research-services/?area=plant) and [food](https://igatechnology.com/agrigenomics-solutions/). Our expertise in the isolation of high molecular weigth DNA for long-molecule sequencing applications will also be a specialized complement for [*de novo*](http://igatechnology.com/genomics-research-services/?area=plant#denovo) applications*.* Pleas [enquire](https://igatechnology.com/igatech/contacts/) to obtain further details*.* ![Metagenomics](/media/images/Metagenomics.width-500.png) ### Metagenomics The area of research which investigates the taxonomical composition and activity of complex biological substrates. While traditional sequencing of cultured microorganisms could not provide a picture of the diversity of natural substrates, shotgun sequencing has opened the possibility of an unbiased fingerprinting and metabolic activity profiling of diverse communities, ranging from soil samples to the human intestinal flora. Several techniques provide scalable solutions. See [metagenomics](https://igatechnology.com/genomics-research-services/plantanimal/metagenomics/) - [metabarcoding](https://igatechnology.com/genomics-research-services/plantanimal/metabarcoding/). ![NovaSeq X Plus](/media/images/Illumina_NovaSeq_XPlus_Left_561x375.width-500.png) ### NovaSeq X Plus The NovaSeq X Plus is a powerful and scalable ultra-high-throughput sequencing system that supports the broadest range of applications and study sizes. Unrivaled data quality using Illumina's proven SBS chemistry has made the NovaSeq X Plus the instrument of choice for all major genome centers and leading institutions throughout the world. Producing up to 25 billion reads \(7.5 Tbp\) per flowcell, yielding up to 120 human genomes in about 2 days at full capacity. ![AVITI Element Biosciences](/media/images/0.width-500.png) ### AVITI Element Biosciences The AVITI System by Element Biosciences is a benchtop sequencer designed for high performance, cost efficiency, and flexibility. It features dual independent flow cells for parallel or independent operation, enabling flexibility in throughput from low to full. The system utilizes Avidity Sequencing™ technology, ensuring over 90% of reads exceed Q30 quality, with minimal errors, low duplication rates, and reduced AT/GC bias. It supports easy data conversion to FASTQ files, offers compatibility with numerous assays, and provides cost-effective sequencing. This is our ideal companion for dedicated and fast-turnaround run set-up. For more detailed information, you can visit their website directly at [https://www.elementbiosciences.com/products/aviti](https://www.elementbiosciences.com/products/aviti). ![Nanopore Sequencing](/media/images/1.width-500.jpg) ### Nanopore Sequencing Nanopore sequencing is empowering many applications by the ability to analyze DNA, RNA and their modifications with no limitation on sequence length. This is of particular importance when the goal is to resolve hard-to-sequence or repetitive regions that have so far hampered the possibility to decode structural and epigenetic variation. Also, direct RNA sequencing and full-length cDNA sequencing are providing unprecedented insight into the real strucure and abundance of transcripts isoforms. **profiling of community-wide gene expression** RNA-seq for identification of both taxonomic composition and active biochemical functions. ### Metatranscriptomics While metagenomics reveals which microbes are present and what potential they have, metatranscriptomics disclose their activity by identifying genes that are expressed in a particular microbial environment. Unbiased **RNA-seq** enables**annotation and quantification** of **transcripts** giving: - **insights in the** **gene activity diversity** and**functional/pathway diversity** by detecting how many genes are expressed in a community across all species - **estimates** **of gene expression abundances** - detects the highest expressed genes in a specific environmental condition, thus **identifying the most important functionality/pathway required** to survive. Note that high sequencing depth is required to detect lower expressed genes - opportunity for **biomarker discovery** by performing a differential gene expression analysis, *i.e.* assessing changes in expression levels between different conditions **Tailored consultancy** For every project we want to make sure that the outcome will meet your expectations **Ribosomal RNA depletion** is performed **for the efficient use of RNA-seq capacity for all samples**. Upon depletion, cDNA library is synthesized, samples are index barcoded and pooled for sequencing. Bioinformatics analysis is directed to determine relative abundance of transcripts/enzymes and pathways for each community. Novel transcripts are assembled and differential expression analysis of different communities is performed in order to identify differentially represented pathways across experimental groups. For all applications final results are delivered in a comprehensive report in addition to raw data and summary of the methods followed. We provide full support on study design to ensure correct sequencing and bioinformatics strategies are used to meet your project goals. Our expert will consult with you about your specific requirements. **Sample RNA extraction** can be part of our workflow. Our **comprehensive sequencing and bioinformatics analysis** services are tailored to your research needs. Choose between receiving raw demultiplexed sequences or a complete bioinformatics analysis with the following key deliverables: ### Raw Reads Service - **Raw Reads with Quality Control Reports**: sequencing data in FASTQ format along with detailed quality control reports. ### Complete Bioinformatics Service - **De Novo Metatranscriptome Assembly**: assembled metatranscriptomes in FASTA format. - **Taxonomic classification**: Accurate assignment from domain to species level - **Predicted Genes and Protein Translations**: predicted genes and their corresponding protein translations in both FASTA and GFF formats, enabling detailed functional analysis. - **Functional Annotations**: Comprehensive data on gene functions and pathways. - **Expression Tables**: raw and normalized expression values for microbial functions quantifying and compareing gene expression levels across samples. - **Differential expression analysis** **of different communities**: identification of differentially represented pathways across experimental groups Enhance your microbiome research with our metatranscriptomics analysis services. Contact us today to learn more about how we can help you uncover the functional mysteries of microbial communities. ## Terms and Conditions - [Privacy Information.pdf](/documents/12/Privacy_Information_rev00.pdf) - [Human samples clearance.pdf](/documents/13/__Human-samples_clearance.pdf) - [Shipping and Packaging Guidelines.pdf](/documents/9/SHIPPING_AND_PACKAGING_GUIDELINES.pdf) - [rev05 Terms and Conditions.pdf](/documents/137/__M12_01_rev05_Terms_and_Conditions.pdf)