--- title: "T2T de novo assembly service" search_description: "IGATech’s de novo assembly services offer precise genome reconstruction for novel species, leveraging long reads, HiC-Seq, and advanced error correction. Ideal for complex genomes in plants, animals, and humans, achieving high-resolution, phased assemblies." published: 2025-01-30 14:41:38.511038+00:00 locale: "it" --- # T2T de novo assembly service ​IGATech has long standing experience in genome assembly, always adopting the latest technologies, protocols and methods. Our mission is to provide consultancy and expertise to our customer in order to plan the optimal and most convenient strategy for de novo assembly projects. [Request a quote](https://igatechnology.com/igatech/contacts/#contact-form) ## Technologies ![PromethION](/media/images/P24.width-500.png) ### PromethION Oxford Nanopore's technology is providing unprecedented accessibility and scalability to long-read sequencing. The technology has proven to be a game-changer in the fields of genome assembly, pangenomics, direct RNA sequencing and metagenomics analyses. Reads can be as long as input DNA/RNA. Bacterial genomes can be assembled in a single molecule in a snap of sequencing. Repetitive elements are easily resolved and with error-correction softwares we can now achieve telomere-to-telomere genome assemblies. ![NovaSeq X Plus](/media/images/Illumina_NovaSeq_XPlus_Left_561x375.width-500.png) ### NovaSeq X Plus The NovaSeq X Plus is a powerful and scalable ultra-high-throughput sequencing system that supports the broadest range of applications and study sizes. Unrivaled data quality using Illumina's proven SBS chemistry has made the NovaSeq X Plus the instrument of choice for all major genome centers and leading institutions throughout the world. Producing up to 25 billion reads \(7.5 Tbp\) per flowcell, yielding up to 120 human genomes in about 2 days at full capacity. ![Nucleic acids extraction](/media/images/DNA_extraction.width-500.png) ### Nucleic acids extraction DNA and RNA extraction is routinely performed at IGATech, providing support to our customers directly from raw material. Relying on automated systems and consolidated protocols we can help you in accelerating the path to analyzed data. We also work with non standard substrates, such as [plants](https://igatechnology.com/genomics-research-services/?area=plant) and [food](https://igatechnology.com/agrigenomics-solutions/). Our expertise in the isolation of high molecular weigth DNA for long-molecule sequencing applications will also be a specialized complement for [*de novo*](http://igatechnology.com/genomics-research-services/?area=plant#denovo) applications*.* Pleas [enquire](https://igatechnology.com/igatech/contacts/) to obtain further details*.* ![Nanopore Sequencing](/media/images/1.width-500.jpg) ### Nanopore Sequencing Nanopore sequencing is empowering many applications by the ability to analyze DNA, RNA and their modifications with no limitation on sequence length. This is of particular importance when the goal is to resolve hard-to-sequence or repetitive regions that have so far hampered the possibility to decode structural and epigenetic variation. Also, direct RNA sequencing and full-length cDNA sequencing are providing unprecedented insight into the real strucure and abundance of transcripts isoforms. ![HiC-Seq](/media/images/2.width-500.jpg) ### HiC-Seq Chromatin interactions are knonw to play an important role in trans-regulatory mechanisms of transcription and chromosome organization. Beside the biologically relevant signals acquired by HiC data \(deviation from expected\), the proximity contact matrix \(expected\) has become a state of-the-art technique to assemble genome at chromosome scale and possibly to phase alterantive alleles to the same extent to delivery complete diploid genome assemblies. ## A cutting-edge toolbox 1. Error correction of raw reads \(Q30+ quality\) 2. Ultra-long reads \(>50Kbp\) 3. Hi-C Scaffolding 4. Extraction of HMW DNA 5. Genome polishing ## Services 1. Pangenome analysis 2. Telomore-to-telomere phased diploid reconstruction 3. Genome anchoring via genetic maps and HiC-Seq 4. Gene prediction and annotation 5. Hosting of private genome browsers ![P24](/media/images/P24.width-1500.png) ## De novo sequencing With the establishment and continuous improvement of NGS technologies, the reconstruction of whole genomes has become an affordable task over the time. Depending on the complexity of genomes, different strategies and technologies can be leveraged to pursue a task that used to require hundreds of thousands of dollars budget; now this task become feasible in the order of few thousands of dollars and less, down to hundreds of dollars for small genomes such as fungi and bacteria. Our commitment is for a continuous research of the best-in-class protocols and bioinformatics solutions to provide our customers gold standard genome assemblies. ![circos](/media/images/circos.width-1500.png) ### The cutting-edge Newer technologies have opened possibilities to improve the reconstruction of complex genomes, such as those of human, animal and plants. The key aspects to pursue nearly complete and exhaustive genome reconstruction is the ability to resolve repeats and distinguish between alleles and/or homolog regions in polyploid species. Such features bring complexity during the assembly procedure which is impossible to disentangle with short sequences alone. At IGATech we have acquired all the technologies and expertise to deliver state-of-art genome assemblies. Among those, **long reads** \(**Oxford Nanopore PromthION**\) and **HiC-Seq protocols**to provide all the resolution to reconstruct**true diploid - phased - assemblies. Thanks to latest base calling models and error correction algorithms raw reads can achieve >99.95% accuracy and unleash the ability to build telomere-to-telomere \(T2T\) chromosomes with a >>99.99% consensus accuracy after short reads polishing.** ## Terms & Conditions - [Privacy Information.pdf](/documents/12/Privacy_Information_rev00.pdf) - [Human samples clearance.pdf](/documents/13/__Human-samples_clearance.pdf) - [Shipping and Packaging Guidelines.pdf](/documents/9/SHIPPING_AND_PACKAGING_GUIDELINES.pdf) - [rev05 Terms and Conditions.pdf](/documents/137/__M12_01_rev05_Terms_and_Conditions.pdf)