---
title: "#LabNote: A new solution for low input miRNA-seq"
search_description: "Deep sequencing of entire population of expressed miRNAs in a given sample simultaneously queries thousands of miRNA sequences with extraordinary sensitivity and ability to detect novel ones. In general, the approach is used to give insights in how post-transcriptional regulation contribute to phenotype and for the discovery and development of novel biomarkers for diagnostic testing. In addition, given its extreme sensitivity, miRNA-seq is suitable for detection of both RNA and DNA viruses, as well as viroids that can be missed using targeted detection methods."
published: 2024-01-16 10:18:15.760469+00:00
locale: "it"
---

# #LabNote: A new solution for low input miRNA-seq

5 Gen 2021

Deep sequencing of entire population of expressed miRNAs in a given sample simultaneously queries thousands of miRNA sequences with extraordinary sensitivity and ability to detect novel ones. In general, the approach is used to give insights in how post-transcriptional regulation contribute to phenotype and for the discovery and development of novel biomarkers for diagnostic testing. In addition, given its extreme sensitivity, miRNA-seq is suitable for detection of both RNA and DNA viruses, as well as viroids that can be missed using targeted detection methods.

**Here is the latest update of our improved miRNA-seq workflow for inputs below 100 ng of total RNA**. We implemented [QIAseq protocol](https://www.qiagen.com/at/products/next-generation-sequencing/rna-sequencing/mirna-small-rnaseq/qiaseq-mirna-ngs/#orderinginformation) indicated for samples with **ultralow input levels** **\(as little as 1 ng of total RNA!**\) to prepare libraries from RNA extracted from several **challenging** **substrates**: grapevine branch tissue, goat milk exosomes and bovine serum. In addition, we also included a sample with very low total RNA quantity originating from mouse fresh frozen tissue.

As shown in the figure below, **despite** **of** **the sample scarcity we were able to obtain a typical miRNA-sized library peak at expected ~180 bp**.

Figure. High Sensitivity DNA Assay 2100 Bioanalyzer traces of miRNA-sized library.

**These results are really encouraging for all of those working with the limited samples such as biofluids \(serum, plasma, CSF, urine\)** **and FFPE tissues**. An **additional advantage** of this particular procedure is that the **kit integrates Unique Molecular Indices \(UMIs\)** during the reverse-transcription process, ensuring that during data analysis **all PCR and sequencing bias are eliminated**.

Hope you found this post useful.

Stay tuned for more news from the NGS world!