Plant and Animal WGS

All genetic tests in one

Whole genome sequencing (WGS) in plants and animals opens a new frontier in genomics, offering transformative possibilities across various fields such as agriculture, breeding, research, and ecology. This comprehensive approach surpasses traditional array-based solutions by providing a depth of information and versatility that can revolutionize how we understand and manipulate the genetic basis of life.

• Farming and Agriculture
• Animal Breeding
• Plant Breeders and Researchers
• Ecology and Conservation

Complete Coverage WGS: This method provides an exhaustive view of the genome, capturing every region with high fidelity. It is ideal for comprehensive genetic analysis, allowing for the detection of both common and rare genetic variants. Complete coverage is essential in clinical research and diagnostics where a detailed understanding of the genetic makeup is crucial for identifying pathogenic mutations and tailoring personalized treatments.

Low-Pass WGS: By sequencing the genome at lower coverage, low-pass WGS offers a cost-effective solution for studies requiring a broad overview rather than detailed variant analysis. This approach is suitable for population genetics and large cohort studies, where the goal is to identify common genetic patterns and associations rather than detailed genotypes of each individual.

Long Reads (Nanopore): Nanopore sequencing provides long reads that can span thousands of base pairs, making it exceptionally useful for characterizing complex regions of the genome, such as structural variants and repetitive sequences. This technology shines in research areas where resolving complex genomic architectures is necessary, such as in the study of genomic rearrangements in cancer or in comprehensive genome assembly projects.

Adaptive Sampling (Nanopore): This advanced feature of Nanopore sequencing technology allows selective sequencing of specific genomic regions of interest. By enriching for particular areas, adaptive sampling makes it possible to focus sequencing efforts and depth where it is most needed, enhancing both cost-efficiency and analytical precision. This approach is particularly beneficial in targeted studies, such as those focusing on specific genes known to be involved in disease, or in regulatory regions critical for gene expression studies.

IGATech Sequencing Services

Our workflow begins with purified genomic DNA or we can provide a dedicated nucleic acids extraction service (please enquire for accepted tissues). PCR-based or PCR-free library preparation can be used to generate libraries.  

Sequencing can be performed on one of the following platforms:

• Illumina NovaSeq
• Element Biosciences AVITI
• Oxford Nanopore PromethION

Several QC steps are performed during the sample processing ensuring the delivery of the highest quality data available.

Bioinformatics Analysis

Genome resequencing - standard bioinformatic analysis includes:

• Alignment to reference sequence
• Quality control and improvement of reads
• Variant calling (germline SNP, indels, SVs)
• Coverage statistics and metrics

Genome resequencing - advanced bioinformatic analysis includes:

• Identification of somatic structural variants
• Identification of somatic SNPs.
• Clonal fingerprinting and parental testing for variety protection
• Identification of chromosomal abnormalities (karyotype testing, aneuploidy, chimeric somatic variants)
• Event-specific insertion analyses

Oxford Nanopore: delivering of phased SNPs, structural variants and methylation profiles in a single experiment

Haplotype-resolved structural variation (Copia-like transposable element insertion on track 1) and associated variation in the methylation pattern

Are you ready to move your genome biology research to the next level?