Ribosome profiling

We provide an innovative solution for ribosome profiling using a fast and selective extraction of ribosomes in active translation (both in vitro and in vivo) and purification of ribosome protected fragments (RPFs). Adopting this new technology may be the key to unlocking new insights into the regulation of ribosomes and changes in the translatome that you've been looking for.

Technologies

NovaSeq6000

RNA-Seq

AVITI Element Biosciences

Features

1
Only active ribosomes captured
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Positional data of active ribosomes with nucleotide resolution
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Rapid protocol to minimize biases

Services

1
Library preparation
2
In-process QC
3
Dedicated 100bp lanes
4
Custom bioinformatics

Exploring the Untold Tales of Translation

Ribosome profiling, also known as ribo-seq, is a powerful experimental technique that provides insights into the translation dynamics and protein synthesis occurring within a cell.

A significant portion of our understanding of gene regulation is derived from research on mRNA levels, even though protein abundance has a greater influence and post-transcriptional regulation is widespread.

Comprehending the molecular fundamentals of regulatory divergence within and between species may be highly advantageous. Most of the recognized human ailment-risk alleles are present in non-coding parts of the genome, implying that they have an influence on gene regulation.

By combining ribosome profiling with mRNA sequencing data, researchers can gain a more comprehensive understanding of gene expression regulation, identify translationally regulated genes, discover novel translation events, and explore the dynamics of protein synthesis within cells.

If you are interested to enter the ribosome-profiling challenges, we are looking forward to help you. Contact us and plan your next experiment with.

Relevant publications

Deciphering the immunopeptidome in vivo reveals new tumour antigens. Nature volume 607, pages149–155 (2022) https://doi.org/10.1038/s41586-022-04839-2

SMN-primed ribosomes modulate the translation of transcripts related to spinal muscular atrophy. Nat Cell Biol. 2020 Oct;22(10):1239-1251. DOI: 10.1038/s41556-020-00577-7

Active Ribosome Profiling with RiboLace. Cell Reports 25, (2018) 1097–1108 DOI: 10.1016/j.celrep.2018.09.084

The science of puromycin: From studies of ribosome function to applications in biotechnology. Comput Struct Biotechnol J. 2020 Apr 24;18:1074-1083. DOI: 10.1016/j.csbj.2020.04.014