Ribosome profiling
We provide an innovative solution for ribosome profiling using a fast and selective extraction of ribosomes in active translation (both in vitro and in vivo) and purification of ribosome protected fragments (RPFs). Adopting this new technology may be the key to unlocking new insights into the regulation of ribosomes and changes in the translatome that you've been looking for.
Request a quoteTechnologies
Features
-
1
Ribosome profiling can be applied to animals, other eukariotes, plants and bacteria
-
2
Starting material can be lysates of flash-frozen tissues or immortalized/primary cell cultures
-
3
Only active ribosomes captured
-
4
Positional data of active ribosomes with nucleotide resolution
-
5
Rapid protocol to minimize biases
Services
-
1
Library preparation
-
2
On-process QC
-
3
Possibility to run dedicated 100bp lanes
-
4
Custom bioinformatics
-
5
Direct collaboration with Immagina Biotech
Exploring the Untold Tales of Translation
Ribosome profiling, also known as ribo-seq, is a powerful experimental technique that provides insights into the translation dynamics and protein synthesis occurring within a cell.
A significant portion of our understanding of gene regulation is derived from research on mRNA levels, even though protein abundance has a greater influence and post-transcriptional regulation is widespread.
Comprehending the molecular fundamentals of regulatory divergence within and between species may be highly advantageous. Most of the recognized human ailment-risk alleles are present in non-coding parts of the genome, implying that they have an influence on gene regulation.
By combining ribosome profiling with mRNA sequencing data, researchers can gain a more comprehensive understanding of gene expression regulation, identify translationally regulated genes, discover novel translation events, and explore the dynamics of protein synthesis within cells.
Until recently, the difficulty of accurately measuring protein abundance inhibited the thorough examination of gene regulation. But, this new technique of ribosome profiling has enabled us to analyze variations in the control of translation.
Relevant pubblications
Deciphering the immunopeptidome in vivo reveals new tumour antigens
Alex M. Jaeger, Lauren E. Stopfer, Ryuhjin Ahn, Emma A. Sanders, Demi A. Sandel, William A. Freed-Pastor, William M. Rideout III, Santiago Naranjo, Tim Fessenden, Kim B. Nguyen, Peter S. Winter, Ryan E. Kohn, Peter M. K. Westcott, Jason M. Schenkel, Sean-Luc Shanahan, Alex K. Shalek, Stefani Spranger, Forest M. White & Tyler Jacks
Nature volume 607, pages149–155 (2022) https://doi.org/10.1038/s41586-022-04839-2
SMN-primed ribosomes modulate the translation of transcripts related to spinal muscular atrophy
Lauria F, Bernabò P, Tebaldi T, Groen EJN, Perenthaler E, Maniscalco F, Rossi A, Donzel D, Clamer M, Marchioretto M, Omersa N, Orri J, Dalla Serra M, Anderluh G, Quattrone A, Inga A, Gillingwater TH, Viero G..
Nat Cell Biol. 2020 Oct;22(10):1239-1251. DOI: 10.1038/s41556-020-00577-7
Active Ribosome Profiling with RiboLace
Massimiliano Clamer, Toma Tebaldi, Fabio Lauria, Paola Bernabo, Rodolfo F. Go mez-Biagi, Marta Marchioretto, Divya T. Kandala, Luca Minati, Elena Perenthaler, Daniele Gubert, Laura Pasquardini, Graziano Guella, Ewout J. N. Groen, Thomas H. Gillingwater, Alessandro Quattrone, Gabriella Viero.
Cell Reports 25, (2018) 1097–1108 DOI: 10.1016/j.celrep.2018.09.084
The science of puromycin: From studies of ribosome function to applications in biotechnology.
Aviner R.
Comput Struct Biotechnol J. 2020 Apr 24;18:1074-1083. DOI: 10.1016/j.csbj.2020.04.014
If you are interested to enter the ribosome-profiling challenges, we are looking forward to help you.