Nanopore phased-methylation

The genomics and epigenomics in one stop shop

The unprecedented ability of Nanopore technology to read native DNA molecules unleash the potential to analyze the genetic and epigenetic code in one single experiment without interfering with the starting DNA template. Molecules of >50Kb can be sequenced to access base content and epigenetic modifications with >99% accuracy.

​Explore Advanced Capabilities with IGATech's Nanopore Sequencing Services

IGATech offers Nanopore Sequencing services, utilizing innovative technology to provide detailed insights into DNA and RNA sequences. This service is pivotal for comprehensive methylation analysis and haplotype reconstruction, enabling researchers to delve into genetic and epigenetic landscapes with precision.

High-Accuracy Methylation Analysis Across All Cytosine Contexts

Our Nanopore Sequencing service excels in detecting methylation across all cytosine contexts (CG, CHG, CHH) with an accuracy of 99%. This high level of precision is crucial for understanding the intricate regulatory mechanisms influenced by epigenetic modifications. The ability to map long reads also in repetitive regions and maintaining a unaltered cytosines (unlike bisulfite or enzymatic conversion of cytosine to T) allow to maintain an extremely high quality of unambiguous mapping and results in complete coverage also of hard-to-sequence regions such as mobile elements, centromers, tandem repeats, etc.

Advanced Bioinformatics Support

IGATech provides advanced bioinformatics support to assist clients in identifying differentially methylated sites and regions, tailoring analyses to specific research objectives. This ensures the extraction of actionable insights from extensive methylation data, including the contextual genomic methylation data across both haplotypes whose phases have been accurately reconstructed thanks to the long reads offered by this technology.

Haplotype-Specific Methylation Information

Our service uniquely resolves haplotypes in diploid individuals, providing haplotype-specific methylation information. This capability allows researchers to examine how methylation patterns differ across chromosomes and assess their impact on gene function and phenotypic traits.

Adaptive Sampling for Targeted Sequencing

IGATech's Nanopore Sequencing also supports adaptive sampling, enabling targeted enrichment of sequencing efforts in regions of high biological interest, such as gene families and their promoters. This feature makes experiments more cost-effective by allowing the sequencing of more samples on the same flow cell, maximizing the utility and efficiency of sequencing runs.

Applications of Nanopore Sequencing Services

Nanopore sequencing has diverse applications across various fields:

• Medical Genetics: Analyze methylation variations and their implications in disease mechanisms.
• Plant and Agricultural Research: Investigate plant methylation to develop crops with enhanced resilience and productivity under diverse environmental conditions.
• Environmental and Ecological Studies: Assess adaptations in organisms to environmental stresses via epigenetic changes.

For more information on how IGATech's Nanopore Sequencing services can support your research needs, please contact our customer service. We provide detailed consultations to integrate our sequencing capabilities into your project specifications, ensuring you receive precise and relevant genetic data.

IGATech is committed to delivering high-quality genomic data through state-of-the-art technologies, enhancing research outcomes across various scientific domains.

We also offer DNA extraction sample preparation service and provide full support on study design to ensure correct sequencing and bioinformatics strategies are used to meet your project goals. Our expert will consult with you about your specific requirements.