Nanopore phased-methylation
Technologies
One experiment - many results
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Accurately call SNPs
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Unprecedented precision in analysis of structural variants
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Define megabase-scale haplotype-blocks
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Achieve haplotype-specific methylation
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Methylation calling in all-context
Specialized bioinformatics
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EPI-haplotype reconstruction
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Methyl and hydroxy-methyl base calls
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Define methylation at and adjacent insertion event
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Genome browser hosting
The genomics and epigenomics in one stop shop
ADAPTIVE SAMPLING
It can enable the in-sequencing enrichment of target regions of interest to enhance the coverage and provide more cost-effective experiments
The unprecedented ability of Nanopore technology to read native DNA molecules unleash the potential to analyze the genetic and epigenetic code in one single experiment without interfering with the starting DNA template. Molecules of >50Kb can be sequenced to access base content and epigenetic modifications with >99% accuracy.
Superior coverage
Thanks to PCR-free preparation and long-reads mapping the a highly homogeneous coverage is achieved without drop in repetitive regions which are usually lost in short-read sequencing. Also centromeric regions can be spanned.
Explore Advanced Capabilities with IGATech's Nanopore Sequencing Services
IGATech offers Nanopore Sequencing services to provide detailed insights into DNA and RNA sequences. This service is pivotal for comprehensive methylation analysis and haplotype reconstruction, enabling researchers to explore genomics landscapes with native data integration..
High-Accuracy Methylation Analysis Across All Cytosine Contexts
With Nanopore Sequencing we can deliver base-by-bae methylation level across all cytosine (CG, CHG, CHH). This high level of precision is crucial for understanding the intricate regulatory mechanisms influenced by epigenetic modifications. The ability to map long reads also in repetitive regions and maintaining unaltered cytosines (unlike bisulfite or enzymatic conversion of cytosine to T) allow to maintain an extremely high quality of unambiguous mapping. Complete and even coverage for hard-to-sequence regions such as mobile elements, centromeres, tandem repeats is the the only approach to complete the picture.
Advanced Bioinformatics Support
IGATech provides advanced bioinformatics support to assist clients in identifying differentially methylated sites and regions, tailoring analyses to specific research objectives. This ensures the extraction of actionable insights from extensive methylation data, including the contextual genomic methylation data across both haplotypes whose phases have been accurately reconstructed thanks to the long reads offered by this technology.
Haplotype-Specific Methylation Information
Our service uniquely resolves haplotypes in diploid individuals, providing haplotype-specific methylation information. This capability allows researchers to examine how methylation patterns differ across chromosomes and assess their impact on gene function and phenotypic traits.
Haplotype-resolved structural variation (Copia-like transposable element insertion on track 1) and associated variation in the methylation pattern
Adaptive Sampling for Targeted Sequencing
IGATech's Nanopore Sequencing also supports adaptive sampling, enabling targeted enrichment of sequencing efforts in regions of high biological interest, such as gene families and their promoters. This feature makes experiments more cost-effective by allowing the sequencing of more samples on the same flow cell, maximizing the utility and efficiency of sequencing runs.
end-to-end solutions
We also offer DNA extraction sample preparation service and provide full support on study design to ensure correct sequencing and bioinformatics strategies are used to meet your project goals. Our expert will consult with you about your specific requirements.