T2T de novo assembly
IGATech has long standing experience in genome assembly, always adopting the latest technologies, protocols and methods. Our mission is to provide consultancy and expertise to our customer in order to plan the optimal and most convenient strategy for de novo assembly projects.
Request a quoteTechnologies
A cutting-edge toolbox
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Error correction of raw reads (Q30+ quality)
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Ultra-long reads (>50Kbp)
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Proximity-reads (HiC-Seq)
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Extraction of HMW DNA
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Short-reads polishing
Services
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Pangenome analysis
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Telomore-to-telomere phased diploid reconstruction
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Genome anchoring via genetic maps and HiC-Seq
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Gene prediction and annotation
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Hosting of private genome browsers
De novo sequencing
With the establishment and continuous improvement of NGS technologies, the reconstruction of whole genomes has become an affordable task over the time. Depending on the complexity of genomes, different strategies and technologies can be leveraged to pursue a task that used to require hundreds of thousands of dollars budget; now this task become feasible in the order of few thousands of dollars and less, down to hundreds of dollars for small genomes such as fungi and bacteria.
Our commitment is for a continuous research of the best-in-class protocols and bioinformatics solutions to provide our customers gold standard genome assemblies.
The cutting-edge
Newer technologies have opened possibilities to improve the reconstruction of complex genomes, such as those of human, animal and plants. The key aspects to pursue nearly complete and exhaustive genome reconstruction is the ability to resolve repeats and distinguish between alleles and/or homolog regions in polyploid species. Such features bring complexity during the assembly procedure which is impossible to disentangle with short sequences alone.
At IGATech we have acquired all the technologies and expertise to deliver state-of-art genome assemblies. Among those, long reads (Oxford Nanopore PromthION) and HiC-Seq protocols to provide all the resolution to reconstruct true diploid - phased - assemblies. Thanks to latest base calling models and error correction algorithms raw reads can achieve >99% accuracy and unleash the ability to build telomere-to-telomere (T2T) chromosomes with a 99.99% consensus accuracy after short reads polishing.