Genotyping by sequencing solutions
by IGA Tech

As the throughput of Illumina sequencing increases, so does its potential as a fast and cost-efficient technology for massive genotyping experiments.
Overcoming the need for expensive and labour-intensive array-based solutions, the redundancy of sequenced reads is used to generate accurate genotyping across large batches of individuals in a single sequencing run.

Solid data output for our clients

Animal and plant breedingAnimal and plant breeding

Genomic information on crops, seeds and livestock allows our clients working in the sector of breeding to make informed selection and breeding decisions. Screening and prediction of useful or disease-linked traits in the organisms help to make crucial decisions on the selection and health of the population.

Biodiversity and nature conservationBiodiversity and nature conservation

Scientists and research institutes in the field of ecology and nature conservation can use GBS solutions to investigate different organisms in their natural habitat, determine the biodiversity of a territory and evaluate the effect of anthropogenic activities. Robust genomic data can also be helpful to trace migration patterns and aid in the regional and international management of the species.

Food industryFood industry

Food producers can use genomic data and our support to assess and certify quality, origin and safety of the ingredients and consequently plan innovative strategies for the traceability of their products. Different GBS solutions provide sensitive detection of biological contamination, ensuring the integrity of any food substrate in the production chain, from raw material to packaged products.

How do we do it? Reducing the DNA to be read

At IGATech, we use different methods to align GBS solutions with budgets. Two of the most effective strategies ddRAD and SPET.

Plant and animal breeding processes, ecology studies and association studies: all these activities usually involve large scale experiments with massive amount of genetic information to sample and analyze. In these cases, whole genome sequencing still remains an unaffordable option.
One of the most effective ways to decrease this cost is to reduce the genome to a fraction of its size before sequencing without losing precious information.

Double digest RAD Sequencing

A technique that uses two restriction enzymes to reduce the complexity of genomes so that we can carry on massive genotyping experiments with very competitive costs.

Single primer enrichment technology

A unique method used for genotyping. It was patented by NuGEN and is employed by IGATech to address large scale genotyping in agrigenomics to deliver uniformly enriched genomic features of organisms with reference sequences.

Advantages: why ddRAD and SPET are the right technologies for our clients

  • Competitive costs. Thanks to ddRAD and SPET, our researchers can develop thousands of genetic markers quickly and effectively at very competitive costs.
  • GBS solutions by IGATech provide solid data. IGATech has successfully conducted many genotyping experiments on both plants and animals, with genome sizes ranging from hundreds of megabases to tens of gigabases. Despite such large differences in genome size and complexity, our researchers managed to obtain effective complexity reduction and to have more than 95% of sequencing data in loci with adequate coverage overall.
  • IGATech provides tailored consultancy, project design and follow-up. We are aware that every genome and experimental design has its own challenges: for every project, we want to make sure that the outcome will meet our customer expectations.
  • No reference DNA sequence needed. Using GBS solutions by IGATech it is possible to genotype organisms that have never been sequenced before.
  • Open approach. Sequencing with ddRAD and SPET allows us to sample genetic variability without bias: this has a critical impact on experiments involving genetic backgrounds not exhaustively represented in an array or any previous experiment.

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